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Interestingly, some cancer cell types and tumor tissues are known to be auxotrophic to specific amino acid(s) [13, 14]

Yes a severe TBI causes anywhere from minor to extensive brain damage, and once a brain cell is dead, we dont have a way to fix it

A clinically meaningful pharmacokinetic interaction would require GHK-Cu to inhibit OCT1, OCT2, or MATE transporters

Proteins were resolved by nonreducing SDSPAGE, and revealed by western blot with 9E10 antiMyc monoclonal (a gift from C Crminon, Saclay, France), antiPdi1 rabbit polyclonal (a gift from J Winther, Denmark), rabbit polyclonal antithioredoxin (a gift from C Grant, UK) or antiHis antibodies, antimouse IgG or antirabbit IgG secondary antibodies labelled with fluorophores of different wavelengths, followed by Infrared Imaging technology (Odyssey, LICOR)